Job ID361186 LocationTwin Cities Job FamilyResearch-Researchers Full/Part TimeFull-Time Regular/TemporaryRegular Job Code8352R1 Employee ClassCivil Service About the Job Summary of Position: The responsibility is assisting PI in performing experiments, collecting data, and summarizing/processing data for publications and grant applications. The essential work includes mouse embryo dissection and a variety of molecular biology and biochemical experiments. The person should integrate the lab's existing data with her/his results and make a summary of each experiment. The person should follow a schedule based on animal experiments and coordinate lab time with other lab members. Essential Duties 20% Mouse embryo dissection, mouse timed mating/plug check (Essential) -Dissect mouse embryos (Stage E8.5 and later) for downstream analysis. -Perform daily plug check with other lab members (7 days a week with weekend rotation). 20% Gene expression analysis in mouse embryo (Essential) -Perform whole mount in situ hybridization of mouse embryos (wild type and mutants). It is essential to identify the exact developmental stage (between E8.5 and E11.5) and to pick up appropriate embryos for experiments. -Take images, record results and summarize data. 20% Immunofluorescence or fluorescent section in situ hybridization and confocal imaging (Essential) -Perform immunofluorescence or in situ hybridization staining of sections and take images by confocal microscope. -Process the images to evaluate gene/protein expression in a quantitative manner. 20% Gene expression analysis (Essential) -Prepare RNA from dissected mouse embryos. -Prepare cDNA and perform qPCR analysis. -Process the results, perform statistical analysis, and summarize data for figures and meeting presentation. 10% Other molecular biology experiments (Secondary) -Acquire the skill for other types of experiments as needed. 10% Assisting the PI (Secondary) -Assist the PI (Dr. Kawakami) with preparation of manuscripts, other documents and figures. -Maintain lab equipment and reagents Qualifications All required qualifications must be documented in application materials Required Qualifications: Bachelor's degree in the biological sciences and successful completion of upper level undergraduate courses in cell biology, genetics, or developmental biology, and basic lab experience, or a combination of education and experience listed to equal 4 years. Good communication skills, and the ability to interact with different levels of researchers (PI, postdoc, lab manager, undergraduates). Preferred Qualifications: Experience in working with mice. Experience in experiments in the area of molecular biology, biochemistry, or developmental biology. About the Department The comprises faculty from both the Medical School and the College of Biological Sciences. Faculty members are engaged in research in three key disciplines of the department-genetics, cell biology and developmental biology. Benefits Working at the University At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work. The University also offers a comprehensive benefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the Low-cost medical, dental, and pharmacy plans Healthcare and dependent care flexible spending accounts University HSA contributions Disability and employer-paid life insurance Employee wellbeing program Excellent retirement plans with employer contribution Public Service Loan Forgiveness (PSLF) Financial counseling services Employee Assistance Program with eight sessions of counseling at no cost with free or reduced rates in the Twin Cities metro area Please visit the for more information re arding benefits. How To Apply Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume. Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section. To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-8647. Diversity The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: Employment Requirements Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment. About the U of M The University of Minnesota, Twin Cities (UMTC) The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations. At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022). The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.